Lu Opera is a local traditional drama in Anhui Province, which was officially approved by the State Council to be included in the first batch of national intangible cultural heritage list in 2006. Lu opera preserves rich original cultural heritage and, with the perseverance and efforts of Lu opera artists throughout history, integrates the characteristics of various relevant arts, showcasing unique historical and cultural value. The study focuses on the cultural factors that influence the singing style of Lu Opera, deeply analyzes the origin and development of Lu Opera, and explores how these factors shape the singing style of Lu Opera from multiple dimensions such as regional ecological culture, economic ecological culture, local folk culture, red revolution culture, and ethical education culture. Research results denote that the singing style of Lu opera is influenced by various cultural factors. The regional ecological culture has shaped the natural expression of Lu opera, the economic ecological culture has influenced the development of its artistic form, the local folk culture has endowed Lu opera with unique regional characteristics, the red revolutionary culture has injected a distinct spirit of the times into Lu opera, and the ethical education culture has deepened the moral connotation of Lu opera. This study not only reveals the diversity and complexity of the singing styles of Lu opera, but also provides theoretical support and practical guidance for the protection and inheritance of this national intangible cultural heritage.