This paper focuses on the combination of vocal level and cultural background in chorus, and points out that in the dimension of emotional interpretation, vocal structure, dynamics and cultural psychological regulation affect the expression of emotion from three aspects: vocal structure, vocal dynamics and cultural psychological regulation. Taking “Yellow River Cantata” and “Bodhisattva Barbarian” as examples, it shows that the association of vocal levels with cultural connotations in different works enriches the form of artistic expression, deepens emotional connotations, and inherits and carries forward the national culture. The combination of sound and cultural context generates the cultural intention in classical poems, reconstructs the cultural context, and depicts the content of the poems and the emotions conveyed by the works. In addition, the integration of original folk songs into modern chorus has an enhancing effect on the manifestation of choral art characteristics, the presentation of cultural symbols and the strengthening of artistic uniqueness. The close combination of sound level and cultural background can enhance the charm of choral art and promote the development and cultural inheritance of choral art.