This study analyzes the role of rural women in building resilient agri-food systems in the department of Boyacá, Colombia, through the adoption of agroecological practices and community leadership strategies. The present study was predicated on a mixed-method approach, which entailed the administration of surveys to 38 producers and the conduction of semi-structured interviews with 15 leaders involved in local sustainable production initiatives. The findings indicate that practices such as crop rotation and association, the utilization of organic fertilizers, effective water management, and the conservation of native seeds play a substantial role in ensuring food security and achieving productive autonomy. Concurrently, limitations associated with restricted land ownership, constrained access to financing, and circumscribed coverage of technical training are identified. The study's findings indicate that the empowerment of women's networks and the proactive integration of their organizations into territorial planning are indispensable for the proliferation and fortification of these practices. The results obtained are consistent with the objectives of Sustainable Development Goals 2, 5, 12, 13, and 15. These results provide a foundation for the formulation of public policies that are designed with a gender focus and territorial relevance.