This study aims to develop and validate a specialized care and prevention protocol to avoid the revictimization of women victims of gender-based violence in the emergency department of the Verdi Cevallos Balda Public Hospital in Portoviejo, Ecuador. Gender-based violence is approached as a structural social and public health problem that transcends individual experiences and is reinforced through institutional practices that may generate secondary and tertiary victimization. The research adopted a qualitative methodology with an action-research design, involving a non-probabilistic convenience sample of eleven multidisciplinary specialists from the health, legal, psychological, and social work fields. Data were collected through documentary analysis, observation, and focus groups, and the proposed protocol was validated using the nominal group technique and Kendall’s coefficient of concordance. The findings reveal a high level of expert consensus regarding the necessity, relevance, and applicability of a context-specific protocol focused on comprehensive, empathetic, and rights-based care. The resulting protocol establishes clear guidelines for medical, psychological, legal, and social intervention, emphasizing institutional coordination, confidentiality, and the prevention of revictimization. The study concludes that adapting national guidelines, such as the “Purple Code,” to local hospital contexts significantly strengthens institutional responses to gender-based violence and contributes to more humane, effective, and gender-sensitive emergency care services.