This paper delves into the profound examination of the conservation and transmission of traditional culture in the context of rural revitalization in Guizhou. It emphasizes the paramount importance of the diverse cultural heritage of Guizhou, vividly highlighting its exceptional richness and incomparable value while concurrently underlining the pressing urgency of bequeathing this invaluable legacy. Furthermore, the paper explores in depth the pivotal role traditional culture plays in rural rejuvenation, encompassing cultural inheritance and societal values. Simultaneously, the article conducts a thorough analysis of the intricate nexus between rural tourism and the preservation of traditional culture, with a particular emphasis on the significant role of rural tourism in cultural continuity. Subsequently, within the backdrop of the present societal milieu, it comprehensively examines the economic, social, and cultural opportunities brought about by rural tourism, alongside the potential commercialization risks, challenges in cultural preservation, and social impacts, among others. Finally, it posits an array of solutions to strike a balance between the economic interests of rural tourism and the imperative need for cultural preservation. The interconnection between Guizhou's rural revitalization and its traditional culture is inseparable, and rural tourism possesses substantial potential for development, yet it necessitates circumspectly addressing an array of challenges. And it is imperative to consider the objectives of cultural inheritance and sustainable development holistically.