The study of innovative expressions and transformation strategies of painting under the perspective of Multi-Source Information Fusion (MSIF) represents a burgeoning research domain. With the continuous advancement of information technology, multi-source information fusion emerges as a pivotal research direction. Within the realm of painting, this integration can provide artists with abundant material and creative inspiration, while also offering viewers a more enriched visual experience. In today's era of informatization and digitization, multi-source information fusion has become a crucial instrument for artistic innovation. Painting, as a unique art form, encounters new opportunities for development when viewed through the lens of multi-source information fusion. By skillfully merging contemporary information technology, painting artists can draw upon a wealth of inspiration, achieving comprehensive innovation. Through multi-source information fusion, various creative methods are explored, not only enhancing the efficiency of painting creation but also expanding the creative space and expressive potential. This paper deeply analyzes, from three different angles—painting creative concepts, painting techniques, and innovative painting forms of expression—the innovative expressions of painting in the perspective of multi-source information fusion, depicting more realistic, three-dimensional, and vibrantly colorful image effects with a greater degree of expressiveness. Finally, based on the characteristics of new painting creative methods, it further explores related transformation strategies and application scenarios to better promote and apply these painting creation methods. This research aims to achieve groundbreaking innovation in painting from the perspective of multi-source information fusion, bearing significant importance in enhancing the expressiveness and perception of painting effects and possessing wide-ranging application value in actual production and creation.