Cultural dance is an important component form of Chinese excellent traditional culture, how to integrate the cultural dance form into the teaching of dance courses has become a hot research topic for the inheritance of national dance culture. This paper establishes a cultural dance course teaching model based on layered O2O teaching, and designs the dance course teaching implementation process from three aspects: before class, during class and after class. A dance course teaching experiment was designed to verify the effects of integrating cultural dance forms into the dance course on the students' dance technique performance, cultural understanding and learning ability, etc. There was a significant difference between the male students' dance professional skill performance in the O2O group and that of the TRD group (P<0.05), and the P values of the female students' daily attendance, technique performance and overall performance were all less than 0.01. 0.01. The cultural understanding of the students in the O2O group improved by 6.25 points at the end of the teaching experiment, and P=0.000<0.01, while the cultural understanding of the students in the TRD group improved by only 0.23 points. Incorporating cultural dance forms into the school dance curriculum can effectively enhance students' cultural comprehension and provide a new path for expanding cultural dance heritage preservation.